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TtRMPVIR
(Plasmid #27995)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27995 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIX (modified)
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8925
  • Vector type
    Mammalian Expression, Retroviral, RNAi ; Fluorescent reporters

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid is low conc., low copy. Use Max efficiency DH5alpha for transformations.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TtRMPVIR (shRen)
  • Alt name
    PMID: 21131983
  • Alt name
    HQ456318
  • Alt name
    miR30 based shRNAs
  • Insert Size (bp)
    116
  • GenBank ID
    HQ456318

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer miR30seq: 5’-tgtttgaatgaggcttcagtac
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TtRMPVIR was a gift from Scott Lowe (Addgene plasmid # 27995 ; http://n2t.net/addgene:27995 ; RRID:Addgene_27995)
  • For your References section:

    Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi. Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe SW. Nat Biotechnol. 2011 Jan . 29(1):79-83. 10.1038/nbt.1720 PubMed 21131983