Addgene - pTGV-CAT SMR5453 Plasmid Data

Plasmid 28033: pTGV-CAT SMR5453

Gene/insert name:
None
Vector backbone:
n/a
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size (bp):
7240
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Chloramphenicol
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Principal Investigator:
Susan Rosenberg
Terms and Licenses:
MTA

Comments:

Note that Addgene's sequencing results found a 1 nucleotide mismatch at bp# 238 and 1 nucleotide deletions at bp# 2970 & 3016 when compared with the sequence provided by the depositing laboratory. These differences are not known to affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome. Gumbiner-Russo et al (Gene. 2001 Jul 25. 273(1):97-104. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 28033" in your Materials and Methods section.