|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28035||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8493
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Note that Addgene's sequencing results found a 1 nucleotide deletion at bp# 4286 when compared with the sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTGV-Express-Tet SMR5918 was a gift from Susan Rosenberg (Addgene plasmid # 28035 ; http://n2t.net/addgene:28035 ; RRID:Addgene_28035)
For your References section:The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome. Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM. Gene. 2001 Jul 25. 273(1):97-104. 10.1016/S0378-1119(01)00565-0 PubMed 11483365