|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28039||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7484
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli.
A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3277. The depositing scientist says this deletion has no impact on the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTGV-Express-CAT SMR5916 was a gift from Susan Rosenberg (Addgene plasmid # 28039 ; http://n2t.net/addgene:28039 ; RRID:Addgene_28039)
For your References section:The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome. Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM. Gene. 2001 Jul 25. 273(1):97-104. 10.1016/S0378-1119(01)00565-0 PubMed 11483365