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Flag-TRIM24
(Plasmid #28138)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28138 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMX-(Flag)x2
  • Backbone manufacturer
    Barton Lab
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRIM24
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • Entrez Gene
    TRIM24 (a.k.a. PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1)
  • Tag / Fusion Protein
    • FLAGX2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer 5’-GTCACACCACAGAAGTAAGGT-3’
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-TRIM24 was a gift from Michelle Barton (Addgene plasmid # 28138 ; http://n2t.net/addgene:28138 ; RRID:Addgene_28138)
  • For your References section:

    Trim24 targets endogenous p53 for degradation. Allton K, Jain AK, Herz HM, Tsai WW, Jung SY, Qin J, Bergmann A, Johnson RL, Barton MC. Proc Natl Acad Sci U S A. 2009 Jul 14. 106(28):11612-6. 10.1073/pnas.0813177106 PubMed 19556538