|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28238||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4731
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth instructionsXL10 gold
Copy numberHigh Copy
Gene/Insert nameHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1)
SpeciesH. sapiens (human)
Insert Size (bp)6306
Entrez GeneNUMA1 (a.k.a. NMP-22, NUMA)
- Promoter CMV
/ Fusion Protein
- EYFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byShuo Dong, Baylor College of Medicine
Terms and Licenses
Article Citing this Plasmid
NuMA insert contains Q772L, E873K and L1575F mutations compared to best match reference sequence (NP_001273490.1).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEYFP-C1-NuMA was a gift from Michael Mancini (Addgene plasmid # 28238)
Generated by Addgene from full sequence.