Gene/insert name: Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1)
Insert size: 6306
Species:
H. sapiens (human)
GenBank ID:
NM_006185
Entrez Gene:
NUMA1
(NUMA)
Fusion protein or tag: EGFP
Terminal: N terminal on backbone
Vector backbone:
pEGFP-C1Search Vector Database )
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4731
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C
List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
XL10 gold
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
Shuo Dong, Baylor College of Medicine
Sequence:
View sequences (2)
Principal Investigator:
Michael Mancini
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
28238" in your Materials and Methods section.
