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pEYFP-C1-NuMA
(Plasmid #28238)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28238 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    XL10 gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6306
  • GenBank ID
  • Entrez Gene
    NUMA1 (a.k.a. NMP-22, NUMA)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

NuMA insert contains Q772L, E873K and L1575F mutations compared to best match reference sequence (NP_001273490.1).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEYFP-C1-NuMA was a gift from Michael Mancini (Addgene plasmid # 28238)