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pEGFP-C1-NuMA
(Plasmid #28238)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    XL10 gold
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6306
  • GenBank ID
    NM_006185
  • Entrez Gene
    NUMA1 (a.k.a. NUMA)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-NuMA was a gift from Michael Mancini (Addgene plasmid # 28238)