pET-TBZ
(Plasmid
#28239)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET17b
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Backbone manufacturerEMD bioscience
- Backbone size w/o insert (bp) 3300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlasmid is provided in DH5a cells. BL21-CodonPlus (DE3)-RILP Competent Cells from Agilent are recommended for expression.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesingle chain Truncated H2B-H2A.Z
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Alt nameTBZ
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)600
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promotor
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-TBZ was a gift from Yawen Bai (Addgene plasmid # 28239 ; http://n2t.net/addgene:28239 ; RRID:Addgene_28239) -
For your References section:
NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. Zhou Z, Feng H, Hansen DF, Kato H, Luk E, Freedberg DI, Kay LE, Wu C, Bai Y. Nat Struct Mol Biol. 2008 Aug . 15(8):868-9. 10.1038/nsmb.1465 PubMed 18641662