pEMS1354
(Plasmid
#29156)
-
PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 29156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEMS1306
- Backbone size w/o insert (bp) 16454
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsDH10B
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePle138
-
Alt nameNR2E1
-
Alt nameOrphan nuclear receptor NR2E1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1996
-
Entrez GeneNR2E1 (a.k.a. TLL, TLX, XTLL)
-
Tag
/ Fusion Protein
- EGFP-NLS (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (unknown if destroyed)
- 3′ cloning site MluI (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarirly final cloning design)
Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by a 6 nucleotide insertion between bp#2169/2170, a 21 nucleotide deletion at bp#116-136 and a 7 nucleotide deletion at bp# 166-17321 nucleotide deletion at bp# 116-136. These differences are not known to affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEMS1354 was a gift from Elizabeth Simpson (Addgene plasmid # 29156 ; http://n2t.net/addgene:29156 ; RRID:Addgene_29156) -
For your References section:
A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM. Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. 10.1073/pnas.1009158107 PubMed 20807748