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Plasmid 29215: pEMS1419
  • Expression of the eGFP reporter gene was not detected in the brain and eye.

  • Ple227

  • THY1

  • Thy-1 membrane glycoprotein precursor

  • 1264

  • H. sapiens (human)

  • THY1 (CD90)

  • EGFP-NLS

  • C terminal on backbone

  • pEMS1306
    (Search Vector Database)

  • Pleiades Promoter Project [sic, Pleaides Plieades]

  • 16454

  • SacI

  • Unknown

  • MluI

  • Unknown

  • Unknown List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • DH10B

  • High Copy

  • View sequences (3)
  • View map

  • pEMS1419 GenBank file (application/octet-stream)

  • pEMS1419 PCR Protocol (application/pdf)

  • Elizabeth Simpson

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please note that Addgene's sequencing results with the EGFP-N primer differ from the full sequence information provided by the depositing laboratory by a 25 nucleotide insertion at bp# 1390/1391. This insertion is not known to affect the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Portales-Casamar et al (Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29215" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with