pEMS1419
(Plasmid #29215)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pEMS1306
  • Backbone size w/o insert (bp) 16454
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Ple227
  • Alt name
    THY1
  • Alt name
    Thy-1 membrane glycoprotein precursor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1264
  • Entrez Gene
    THY1 (a.k.a. CD90)
  • Tag / Fusion Protein
    • EGFP-NLS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that Addgene's sequencing results with the EGFP-N primer differ from the full sequence information provided by the depositing laboratory by a 25 nucleotide insertion at bp# 1390/1391. This insertion is not known to affect the function of the plasmid.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1419 was a gift from Elizabeth Simpson (Addgene plasmid # 29215)
  • For your References section:

    A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J, Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM. Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. 10.1073/pnas.1009158107 PubMed 20807748