Addgene - pEMS1673 Plasmid Data

Plasmid 29290: pEMS1673

Gene/insert name:
Ple201
Alt name:
SLC6A3
Alt name:
solute carrier family 6
Insert size:
1994
Species:
H. sapiens (human)
Entrez Gene:
SLC6A3 (DAT, DAT1, PKDYS)
Fusion protein or tag:
Intron-LacZ-NLS
Terminal:
C terminal on backbone
Vector backbone:
pEMS1313
(Search Vector Database)
Vector type:
Pleiades Promoter Project [sic, Pleaides Plieades]
Backbone size w/o insert (bp):
18843
Cloning site 5':
FseI
Site destroyed during cloning:
Unknown
Cloning site 3':
AscI
Site destroyed during cloning:
Unknown
5' sequencing primer:
Unknown List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH10B
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Elizabeth Simpson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Portales-Casamar et al (Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29290" in your Materials and Methods section.

Price: $65.00

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