Addgene - pTH442-SUP45-K176T Plasmid Data

Plasmid 29385: pTH442-SUP45-K176T

Gene/insert name:
SUP45 gene encoding translation release factor 1 from S. cerevisiae
Alt name:
eRF1
Insert size:
2984
Species:
S. cerevisiae (budding yeast)
GenBank ID:
NM_001178491
Entrez Gene:
SUP45 (YBR143C, SAL4, SUP1, SUP47)
Mutation:
K176T mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon.
Vector backbone:
pRS315
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5976
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
LEU2
Sequence: View sequences (1)
Principal Investigator:
Tobias von der Haar
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast. Merritt et al (Nucleic Acids Res. 2010 Sep . 38(16):5479-92. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29385" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with