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pBacPAK8-His-VHL-Flag(117-213)
(Plasmid #29501)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 29501 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBacPAK8
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Von Hippel-Lindau
  • Alt name
    VHL
  • Species
    H. sapiens (human)
  • Mutation
    VHL(117-213) only
  • Entrez Gene
    VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site don't know (unknown if destroyed)
  • 3′ cloning site don't know (unknown if destroyed)
  • 5′ sequencing primer Bac1 Primer
  • 3′ sequencing primer Bac2 Primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBacPAK8-His-VHL-Flag(117-213) was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 29501 ; http://n2t.net/addgene:29501 ; RRID:Addgene_29501)
  • For your References section:

    Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex. Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW. Proc Natl Acad Sci U S A. 2000 Sep 12. 97(19):10430-5. 10.1073/pnas.190332597 PubMed 10973499