Addgene - pBacPAK8-His-VHL-Flag(117-213) Plasmid Data

Plasmid 29501: pBacPAK8-His-VHL-Flag(117-213)

Gene/insert name:
Von Hippel-Lindau
Alt name:
VHL
Species:
H. sapiens (human)
Entrez Gene:
VHL (HRCA1, RCA1, VHL1, pVHL)
Fusion protein or tag:
His
Terminal:
N terminal on insert
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Mutation:
VHL(117-213) only
Vector backbone:
pBacPAK8
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Insect Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
don't know
Site destroyed during cloning:
Unknown
Cloning site 3':
don't know
Site destroyed during cloning:
Unknown
5' sequencing primer:
Bac1 Primer List of Sequencing Primers
3' sequencing primer:
Bac2 Primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Joan Conaway
Principal Investigator:
Ronald Conaway
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex. Kamura et al (Proc Natl Acad Sci U S A. 2000 Sep 12. 97(19):10430-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29501" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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