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pSG5-CRKL
(Plasmid #29560)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29560 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRKL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_005207
  • Entrez Gene
    CRKL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SMAI (destroyed during cloning)
  • 3′ cloning site SMAI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Crkl pSG5. This construct encodes the human adapter protein Crkl. Crkl is a protein that contains one SH2 domain and two SH3 domains. It can become tyrosine phosphorylated by the c-Abl tyrosine kinase on residue Y207. Crkl is located on chromosome 22q11.21. Gene ID: 1399. HPRD: 03596. NM_005207.
The construct was made by Joanne ten Hoeve in 1992/1993.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-CRKL was a gift from Nora Heisterkamp (Addgene plasmid # 29560 ; http://n2t.net/addgene:29560 ; RRID:Addgene_29560)
  • For your References section:

    CRK proto-oncogene maps to human chromosome band 17p13. Fioretos T, Heisterkamp N, Groffen J, Benjes S, Morris C. Oncogene. 1993 Oct . 8(10):2853-5. PubMed 8378094