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SEMA3A (chick) pBLUE
(Plasmid #29564)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29564 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBLUESCRIPT (SK)
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    pBlue for ISH

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEMA3A (chick)
  • Alt name
    Collapsin 1
  • Alt name
    Semaphorin 3A
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2100
  • Mutation
    2.1kb fragment for in situ hybridization (ISH)
  • GenBank ID
    NM_204977
  • Entrez Gene
    SEMA3A (a.k.a. COLL-1, semaphorin-3A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase.

Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SEMA3A (chick) pBLUE was a gift from Jonathan Raper (Addgene plasmid # 29564 ; http://n2t.net/addgene:29564 ; RRID:Addgene_29564)