SEMA3A (chick) pBLUE
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29564||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBLUESCRIPT (SK)
- Backbone size w/o insert (bp) 3000
Vector typepBlue for ISH
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSEMA3A (chick)
Alt nameCollapsin 1
Alt nameSemaphorin 3A
SpeciesG. gallus (chicken)
Insert Size (bp)2100
Mutation2.1kb fragment for in situ hybridization (ISH)
Entrez GeneSEMA3A (a.k.a. COLL-1, semaphorin-3A)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase.
Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SEMA3A (chick) pBLUE was a gift from Jonathan Raper (Addgene plasmid # 29564 ; http://n2t.net/addgene:29564 ; RRID:Addgene_29564)