Addgene - Map3k1 Plasmid Data

Plasmid 29581: Map3k1

Gene/insert name:
Map3k1
Species:
H. sapiens (human)
Entrez Gene:
MAP3K1 (MAPKKK1, MEKK, MEKK 1, MEKK1, SRXY6)
Fusion protein or tag:
HIS6
Terminal:
N terminal on backbone
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Fusion protein or tag:
TEV
Terminal:
N terminal on backbone
Mutation:
Constitutively Active. Contains catalytic domain D874-end.
Vector backbone:
pMCSG10
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5961
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Dustin Maly
Terms and Licenses:
MTA

Comments:

Ligation-independent-cloning.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29581" in your Materials and Methods section.