|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29585||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typezebrafish expression
Growth in Bacteria
Growth instructionsstbl3 @ 30 degrees
Insert Size (bp)8700
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
mpx is a promoter for neutrophil expression and was called zMPO in the associated publication
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:tol2-mpx-mCherry was a gift from Anna Huttenlocher (Addgene plasmid # 29585 ; http://n2t.net/addgene:29585 ; RRID:Addgene_29585)
For your References section:Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish. Yoo SK, Deng Q, Cavnar PJ, Wu YI, Hahn KM, Huttenlocher A. Dev Cell. 2010 Feb 16. 18(2):226-36. 10.1016/j.devcel.2009.11.015 PubMed 20159593