pENTR4-SB1-LUC (w832-1)
(Plasmid #29647)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pENTR4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2277
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1652
  • Tag / Fusion Protein
    • SB1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The firefly luciferase cDNA is derived from the plasmid pGL3 (Promega).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR4-SB1-LUC (w832-1) was a gift from Eric Campeau (Addgene plasmid # 29647)