pTH490-tQSRTL
(Plasmid #29699)

• Depositing Lab: Tobias von der Haar
• Publication: Chu et al Nucleic Acids Res. 2011 May 9. ():.

Backbone

• Vector backbone: pRS315
• Backbone size w/o insert (bp): 5954
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G
• Alt name: Low abundance arginyl-; glutaminyl-; threonyl-; seryl- and leucyl tRNA genes.
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1493

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EclXI (not destroyed)
• 5′ sequencing primer: M13f (-40)
• 3′ sequencing primer: M13r (-48)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a single nucleotide (A) deletion in the sequence. This deletion does not affect the function of the plasmid.