HL 3545 (=HL 770 + pHL 1213)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30081||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneColE (from pZ system) + AmpR
Backbone manufacturerLim Lab (modified from Rolf Lutz and Hermann Bujard, 1997)
- Backbone size w/o insert (bp) 2000
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Growth Strain(s)HL 770
Copy numberHigh Copy
Gene/Insert namepLlacO-1:hfq, pLtetO-1:RBS (st7) gfp
Insert Size (bp)2100
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site AatII (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer ctcatgagcggatacat atttgaa
- 3′ sequencing primer agctgatacc gctcgccgca gccgaacg (Common Sequencing Primers)
Terms and Licenses
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HL 3545 (=HL 770 + pHL 1213) was a gift from Han Lim (Addgene plasmid # 30081 ; http://n2t.net/addgene:30081 ; RRID:Addgene_30081)
For your References section:Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298