pCAX FLAG APP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30154||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAmyloid Precursor Protein
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneAPP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, preA4)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAX FLAG APP was a gift from Dennis Selkoe & Tracy Young-Pearse (Addgene plasmid # 30154 ; http://n2t.net/addgene:30154 ; RRID:Addgene_30154)
For your References section:A critical function for beta-amyloid precursor protein in neuronal migration revealed by in utero RNA interference. Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ. J Neurosci. 2007 Dec 26. 27(52):14459-69. 10.1523/JNEUROSCI.4701-07.2007 PubMed 18160654