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pAd-TNT-rtTA-IRES-BirA
(Plasmid #30215)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 30215 Plasmid sent as bacteria in agar stab 1 $65 Add to Cart
Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pAd-TNT-rtTA-IRES-BirA
  • Insert Size (bp)
    33000

Cloning Information

Resource Information

Depositor Comments

Expresses rtTA and BirA from the cardiac troponin T promoter. BirA biotinylates the Bio tag.

Please note that Addgene has been unable to sequence this plasmid. Users may wish to sequence a PCR product generated from the bacteria using primers listed with the sequencing results from the depositing scientist [see View Sequences link above].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-TNT-rtTA-IRES-BirA was a gift from William Pu (Addgene plasmid # 30215)
  • For your References section:

    Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. He A, Kong SW, Ma Q, Pu WT. Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7. 10.1073/pnas.1016959108 PubMed 21415370