|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30462||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3812
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHNF1a promoter
Alt nameLFB1 promoter
SpeciesX. laevis (frog)
Insert Size (bp)5700
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
The GFP variant in the plasmid backbone, pCSGFP2, contains the F64L and the S65T mutations resulting in 35 times brighter green emission (as described in Ikawa M et al., Curr. Top. Dev. Biol. 44 (1999), pp. 1–20)
This construct is derived from the (−6 kb/−58)-GFP construct [Addgene plasmid number 30460] by replacing the EcoRI–HindIII fragment (−494 to −58) with the corresponding EcoRI–HindIII fragment (−494 to −207) of the 3Δ207 clone (as described in Zapp D et al., Mol. Cell. Biol. 13 (1993), pp. 6416–6426).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:(-6kb/-207)-GFP was a gift from Gerhart Ryffel (Addgene plasmid # 30462 ; http://n2t.net/addgene:30462 ; RRID:Addgene_30462)
For your References section:Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus. Ryffel GU, Lingott A. Mech Dev. 2000 Jan . 90(1):65-75. 10.1016/S0925-4773(99)00230-0 PubMed 10585563