|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30525||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGenbank DQ836146
- Backbone size w/o insert (bp) 3007
Vector typeMammalian Expression, Cre/Lox ; heat shock inducible, I-SceI Megabase vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCre recombinase tdTomato
Speciessynthetic, bacteriophage P1
Insert Size (bp)4000
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Cre driven by Xenopus laevis heat shock 70 (hsp 70) promoter with CMV driven tdTomato, both in I-SceI megabase vector
Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. These discrepancies are not known to affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBSHSP:Cre;CMV:tdTomato-SceI was a gift from Gerhart Ryffel (Addgene plasmid # 30525 ; http://n2t.net/addgene:30525 ; RRID:Addgene_30525)
For your References section:Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis. Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, Senkel S, Ryffel GU, Waldner C. Transgenic Res. 2009 Aug . 18(4):595-605. 10.1007/s11248-009-9253-4 PubMed 19266305