|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30538||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeWorm Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsRequires growth in a strain resistant to ccdB (DB3.1, ccdB Survival, or F')
Copy numberHigh Copy
Gene/Insert namettTi4348 region
SpeciesC. elegans (nematode); C. briggsae
Insert Size (bp)5200
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Unpublished MosSCI reagents from the Jorgensen lab.
This vector targets the site ttTi4348 on Chr. I.
Use with strain EG6701 at the CGC.
Note - plasmid contains TWO M13F primer sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ210 was a gift from Erik Jorgensen (Addgene plasmid # 30538 ; http://n2t.net/addgene:30538 ; RRID:Addgene_30538)