Addgene - pBS-F2-GTC Plasmid Data

Plasmid 30728: pBS-F2-GTC

Gene/insert name:
F2 GTC
Insert size:
276
Vector backbone:
pBlueScript II SK(+)
(Search Vector Database)
Vector type:
Cloning vector
Backbone size w/o insert (bp):
2877
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
XbalI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
XL1Blue
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Zhu et al (Development. 2011 Oct;138(20):4555-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30728" in your Materials and Methods section.