Addgene - pSuper-TatSF1 AB Plasmid Data

Plasmid 31119: pSuper-TatSF1 AB

Gene/insert name:
TatSF1 shRNA
Alt name:
TatSF1
shRNA sequence:
GAGTCAGATGACAAGGAAG
Species:
H. sapiens (human)
Entrez Gene:
HTATSF1 (RP1-196E23.2, TAT-SF1, dJ196E23.2)
Vector backbone:
pSuper
(Search Vector Database)
Backbone manufacturer:
Oligoengine
Vector type:
Mammalian Expression, RNAi
Backbone size w/o insert (bp):
3176
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Mariano Garcia-Blanco
Terms and Licenses:
MTA

Comments:

shRNA TatSF1 Target: GAGTCAGATGACAAGGAAG

NB: This shRNA for TatSF1 and Plasmid #31120 both work very well.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tat-SF1 is not required for Tat transactivation but does regulate the relative levels of unspliced and spliced HIV-1 RNAs. Miller et al (PLoS One. 2009 . 4(5):e5710. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31119" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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