Addgene - 782 pMEActMEKK Plasmid Data

Plasmid 31166: 782 pMEActMEKK

Gene/insert name:
MEKK1
Species:
H. sapiens (human)
Entrez Gene:
MAP2K1 (MAPKK1, MEK1, MKK1, PRKMK1)
Mutation:
Ser218Glu Ser222Asp; activated MEKK
Vector backbone:
pME
(Search Vector Database)
Vector type:
Entry vector
Backbone size w/o insert (bp):
2503
Cloning method:
Gateway Cloning
Cloning site 5':
Gateway
Site destroyed during cloning:
No
Cloning site 3':
Gateway
Site destroyed during cloning:
No
5' sequencing primer:
M13pUC-fwd List of Sequencing Primers
3' sequencing primer:
M13rev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

Middle entry clone.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development. Covassin et al (Dev Biol. 2009 May 15. 329(2):212-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31166" in your Materials and Methods section.