Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31232||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK(+)
- Backbone size w/o insert (bp) 3000
Vector typecDNA for in situ hybridization
Growth in Bacteria
Copy numberHigh Copy
Alt nameclass III B-tubulin
SpeciesG. gallus (chicken)
Insert Size (bp)434
MutationC-terminal + 3'UTR sequence
- Cloning method Restriction Enzyme
- 5′ cloning site BstZ1 (unknown if destroyed)
- 3′ cloning site SmaI (unknown if destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For riboprobes, cut with EagI. Runoffs with T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcTUBB3 was a gift from Cliff Ragsdale (Addgene plasmid # 31232 ; http://n2t.net/addgene:31232 ; RRID:Addgene_31232)
For your References section:Arcuate plan of chick midbrain development. Sanders TA, Lumsden A, Ragsdale CW. J Neurosci. 2002 Dec 15. 22(24):10742-50. PubMed 12486167