Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31235||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3950
Vector typecDNA for in situ hybridization
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
SpeciesG. gallus (chicken)
Insert Size (bp)631
- Cloning method TOPO Cloning
- 5′ cloning site TOPO (unknown if destroyed)
- 3′ cloning site TOPO (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDISP1 was a gift from Cliff Ragsdale (Addgene plasmid # 31235 ; http://n2t.net/addgene:31235 ; RRID:Addgene_31235)
For your References section:Differential susceptibility of midbrain and spinal cord patterning to floor plate defects in the talpid2 mutant. Agarwala S, Aglyamova GV, Marma AK, Fallon JF, Ragsdale CW. Dev Biol. 2005 Dec 1. 288(1):206-20. 10.1016/j.ydbio.2005.09.034 PubMed 16246323