Holiday Schedule: Addgene will be closed December 22nd & 25th and December 29th & January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pDM#834
(Plasmid #31240)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31240 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD95.75
  • Backbone manufacturer
    Andrew Fire (Addgene plasmid# 1494)
  • Backbone size (bp) 7980
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Species
    C. elegans (nematode)
  • Tag / Fusion Protein
    • GST (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

IMPORTANT: Though the map depicts suggests that the CAT/CamR gene is present, this plasmid does NOT confer Chloramphenicol resistance. That gene is a remnant of the cloning and is not functional.

From the depositor: "The clone is a GFP fusion vector that carries a C. elegans muscle specific promoter in front of the GFP sequence as a pDEST destination vector. Protein coding sequences are introduced into this vector between the promoter and GFP sequences by recombination from a pDonor clone using the Invitrogen clonase kit. The backbone vector was pPD95_75 and the promoter and pDEST recombination sequences were cloned into this vector."

Please note that there are discrepancies between the author's sequence and Addgene's quality control sequence, but the depositing lab does not believe these differences should affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDM#834 was a gift from Don Moerman (Addgene plasmid # 31240)
  • For your References section:

    Determining the Sub-Cellular Localization of Proteins within Caenorhabditis elegans Body Wall Muscle. Meissner B, Rogalski T, Viveiros R, Warner A, Plastino L, Lorch A, Granger L, Segalat L, Moerman DG. PLoS One. 2011 . 6(5):e19937. 10.1371/journal.pone.0019937 PubMed 21611156