|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31240||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAndrew Fire (Addgene plasmid# 1494)
- Backbone size (bp) 7980
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
/ Fusion Protein
- GST (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
IMPORTANT: Though the map depicts suggests that the CAT/CamR gene is present, this plasmid does NOT confer Chloramphenicol resistance. That gene is a remnant of the cloning and is not functional.
From the depositor: "The clone is a GFP fusion vector that carries a C. elegans muscle specific promoter in front of the GFP sequence as a pDEST destination vector. Protein coding sequences are introduced into this vector between the promoter and GFP sequences by recombination from a pDonor clone using the Invitrogen clonase kit. The backbone vector was pPD95_75 and the promoter and pDEST recombination sequences were cloned into this vector."
Please note that there are discrepancies between the author's sequence and Addgene's quality control sequence, but the depositing lab does not believe these differences should affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDM#834 was a gift from Don Moerman (Addgene plasmid # 31240)
For your References section:Determining the Sub-Cellular Localization of Proteins within Caenorhabditis elegans Body Wall Muscle. Meissner B, Rogalski T, Viveiros R, Warner A, Plastino L, Lorch A, Granger L, Segalat L, Moerman DG. PLoS One. 2011 . 6(5):e19937. 10.1371/journal.pone.0019937 PubMed 21611156