Addgene - FR_GFP Plasmid Data

Plasmid 31302: FR_GFP

Gene/insert name:
green fluorescent protein
Alt name:
GFP
Insert size:
700
Vector backbone:
pcDNA5/FRT/TO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5137
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Gerhart Ryffel
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

for Flp mediated site directed integration, tetracycline inducibility

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tissue-specific transcription factor HNF4alpha inhibits cell proliferation and induces apoptosis in the pancreatic INS-1 beta-cell line. Erdmann et al (Biol Chem. 2007 Jan . 388(1):91-106. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31302" in your Materials and Methods section.