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L4440-daf-16b
(Plasmid #31504)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    daf-16b specific
  • gRNA/shRNA sequence
    see View Sequences link below
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    531
  • Entrez Gene
    daf-16 (a.k.a. CELE_R13H8.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer M13-F20
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Please note that Addgene's sequencing results found two nucleotide mismatches at bp# 450 & 525 when compared to the sequence provided by the depositing scientist. These mismatches are not a concern for the function of the plasmid.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4440-daf-16b was a gift from Heidi Tissenbaum (Addgene plasmid # 31504)
  • For your References section:

    A new DAF-16 isoform regulates longevity. Kwon ES, Narasimhan SD, Yen K, Tissenbaum HA. Nature. 2010 Jul 22. 466(7305):498-502. 10.1038/nature09184 PubMed 20613724