Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31504||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2790
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namedaf-16b specific
gRNA/shRNA sequencesee View Sequences link below
SpeciesC. elegans (nematode)
Insert Size (bp)531
Entrez Genedaf-16 (a.k.a. CELE_R13H8.1)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
Please note that Addgene's sequencing results found two nucleotide mismatches at bp# 450 & 525 when compared to the sequence provided by the depositing scientist. These mismatches are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:L4440-daf-16b was a gift from Heidi Tissenbaum (Addgene plasmid # 31504)
For your References section:A new DAF-16 isoform regulates longevity. Kwon ES, Narasimhan SD, Yen K, Tissenbaum HA. Nature. 2010 Jul 22. 466(7305):498-502. 10.1038/nature09184 PubMed 20613724