1
Plasmid 31504: L4440-daf-16b
  • daf-16b specific

  • 531

  • see View Sequences link below

  • C. elegans (nematode)

  • daf-16 (CELE_R13H8.1)

  • L4440
    (Search Vector Database)

  • RNAi

  • 2790

  • HindIII

  • No

  • NheI

  • No

  • NA List of Sequencing Primers

  • M13-F20

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • View sequences (2)
  • Heidi Tissenbaum

  • UBMTA

Comments: 

Please note that Addgene's sequencing results found two nucleotide mismatches at bp# 450 & 525 when compared to the sequence provided by the depositing scientist. These mismatches are not a concern for the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A new DAF-16 isoform regulates longevity. Kwon et al (Nature. 2010 Jul 22. 466(7305):498-502. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31504" in your Materials and Methods section.