Addgene - L4440-daf-16b Plasmid Data

Plasmid 31504: L4440-daf-16b

Gene/insert name:
daf-16b specific
Insert size:
531
shRNA sequence:
see View Sequences link below
Species:
C. elegans (nematode)
Entrez Gene:
daf-16 (CELE_R13H8.1)
Vector backbone:
L4440
(Search Vector Database)
Vector type:
RNAi
Backbone size w/o insert (bp):
2790
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
NheI
Site destroyed during cloning:
No
5' sequencing primer:
NA List of Sequencing Primers
3' sequencing primer:
M13-F20
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Heidi Tissenbaum
Terms and Licenses:
MTA

Comments:

Please note that Addgene's sequencing results found two nucleotide mismatches at bp# 450 & 525 when compared to the sequence provided by the depositing scientist. These mismatches are not a concern for the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A new DAF-16 isoform regulates longevity. Kwon et al (Nature. 2010 Jul 22. 466(7305):498-502. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31504" in your Materials and Methods section.