Addgene - GFP-PICK Plasmid Data

Plasmid 31613: GFP-PICK

Gene/insert name:
PICK1 (shRNA insensitive) +shRNA towards PICK1
Alt name:
PICK1
Species:
R. norvegicus (rat)
Entrez Gene:
Pick1 (Prkcabp)
Fusion protein or tag:
GFP
Terminal:
N terminal on insert
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Fusion protein or tag:
PICK1 shRNA
Terminal:
N terminal on backbone
Vector backbone:
FUGW
(Search Vector Database)
Backbone manufacturer:
David Baltimore (Caltech)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9941
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Robert Malenka
Terms and Licenses:
MTA

Comments:

This is the replacement construct which expresses an shRNA to knockdown the endogenous protein and a recombinant GFP-tagged, shRNA-resistant protein, to replace the endogenous PICK1.

shRNA sequence: CTATGAGTACCGCCTTATCCT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression. Citri et al (J Neurosci. 2010 Dec 8. 30(49):16437-52. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31613" in your Materials and Methods section.