pT3-EF1a-c-Met
(Plasmid #31784)

• Depositing Lab: Xin Chen
• Publication: Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep 4.

Backbone

• Vector backbone: pT3-EF1a
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Slow growing plasmids, grow at least 18 hours.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Met
• Species: H. sapiens (human)
• Insert Size (bp): 4173
• Entrez Gene: MET (a.k.a. AUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met)
• Promoter: EF1alpha

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF-1a-Fwd

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.