pJRT.Agro.CLCrVA.008
(Plasmid #31809)

• Depositing Lab: Niki Robertson
• Publication: Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.

Backbone

• Vector backbone: pCAMBIA 1300
• Backbone manufacturer: Cambia Labs
• Backbone size w/o insert (bp): 8935
• Total vector size (bp): 11443
• Modifications to backbone: CLCrV VIGS A component empty vector was inserted between the SacI and SalI sites in the pCAMBIA 1300 backbone.
• Vector type: Agrobacterium Binary
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JRTCLCrVA.008
• Alt name: JRT.008
• Alt name: CLCrVA:Empty Vector
• Species: Begomovirus
• Insert Size (bp): 2508
• GenBank ID: EU541444.1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: AACAGCTATGACCATG
• 3′ sequencing primer: GTAAAACGACGGCCAGT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results have detected an IS4 family transposase upstream of the kanamycin resistance cassette. This element had no effect on virus-induced gene silencing of genes in cotton in the depositing lab. However, the concentration of kanamycin for selection may need to be reduced from 50 to 25 mg/l.