|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31831||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3216
Vector typeCloning vector
Growth in Bacteria
Gene/Insert nameTol2 Transposase
Insert Size (bp)1952
- Promoter Lac
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer Lac_promoter
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT3TS-Tol2 was a gift from Stephen Ekker (Addgene plasmid # 31831)
For your References section:Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.