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pT3TS-Tol2
(Plasmid #31831)

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    Tol2 Transposase
  • Species
    Artificial sequence
  • Insert Size (bp)
    1952
  • Promoter Lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer Lac_promoter
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3TS-Tol2 was a gift from Stephen Ekker (Addgene plasmid # 31831)
  • For your References section:

    Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.