pIRESpuro2-UBXD1-3x HA
(Plasmid #31836)

• Depositing Lab: Hemmo Meyer
• Publication: Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23. doi: 10.1038/ncb2301.

Backbone

• Vector backbone: pIRESpuro2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBXD1
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• GenBank ID: NP_079517
• Entrez Gene: UBXN6 (a.k.a. UBXD1, UBXDC2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3x HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UBXD1 cloned into the pIRESpuro2 vector with 5'EcoRI-BamHI3' and 3x HA tag cloned separately with 5'BamHI-NotI3'.