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pALACE
(Plasmid #31853)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31853 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACE
  • Backbone manufacturer
    Koen De Smet Imperial College Medical School
  • Backbone size (bp) 8221
  • Vector type
    Bacterial Expression ; Escherichia coli-Mycobacterium shuttle plasmid
  • Promoter Acetamidase operone
  • Tag / Fusion Protein
    • 6xHIS (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

  • Terms and Licenses

Depositor Comments

Plasmid can also be grown in mycobacteria.

Also see PMID 15342574 for additional reference.

Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pALACE was a gift from Yossef Av-Gay (Addgene plasmid # 31853 ; http://n2t.net/addgene:31853 ; RRID:Addgene_31853)
  • For your References section:

    The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA). Newton GL, Koledin T, Gorovitz B, Rawat M, Fahey RC, Av-Gay Y. J Bacteriol. 2003 Jun;185(11):3476-9. 10.1128/JB.185.11.3476-3479.2003 PubMed 12754249