Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31853||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerKoen De Smet Imperial College Medical School
- Backbone size (bp) 8221
Vector typeBacterial Expression ; Escherichia coli-Mycobacterium shuttle plasmid
- Promoter Acetamidase operone
/ Fusion Protein
- 6xHIS (N terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
Terms and Licenses
Plasmid can also be grown in mycobacteria.
Also see PMID 15342574 for additional reference.
Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pALACE was a gift from Yossef Av-Gay (Addgene plasmid # 31853 ; http://n2t.net/addgene:31853 ; RRID:Addgene_31853)
For your References section:The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA). Newton GL, Koledin T, Gorovitz B, Rawat M, Fahey RC, Av-Gay Y. J Bacteriol. 2003 Jun;185(11):3476-9. 10.1128/JB.185.11.3476-3479.2003 PubMed 12754249
Map uploaded by the depositor.