Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31883||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTGFBR2 mutant 3'UTR
SpeciesH. sapiens (human)
MutationThird miR-302b, 372 7-mer seed match mutated from AGCACTT to AGAAATT
- Promoter SV40
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer psiCHECK2-F (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psiCHECK-2-TGFBR2-m3_3'UTR was a gift from Robert Blelloch (Addgene plasmid # 31883 ; http://n2t.net/addgene:31883 ; RRID:Addgene_31883)
For your References section:Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells. Subramanyam D, Lamouille S, Judson RL, Liu JY, Bucay N, Derynck R, Blelloch R. Nat Biotechnol. 2011 May;29(5):443-8. Epub 2011 Apr 13. 10.1038/nbt.1862 PubMed 21490602