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Plasmid 31900: pMT-Pygopus
  • Pygopus

  • D. melanogaster (fly)

  • pygo (Dmel_CG11518, BEST:LD21971, CG11518, Dm Pygo, Dmel\CG11518, Pygo, anon-WO02077023.1, dPygo, gam, pNB5.2, pyg, pypo)

  • V5

  • C terminal on backbone

  • 6xHIS

  • C terminal on backbone

  • pMT-V5/HIS
    (Search Vector Database)

  • Invitrogen

  • Insect Expression

  • 3538

  • P-MT (Metallothionein)

  • none

  • Unknown

  • none

  • Unknown

  • Metallothionein List of Sequencing Primers

  • EBV reverse

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • See comments

  • View sequences (1)
  • Robert Tjian

  • UBMTA

Comments: 

Blasticidin OR Hygromycin selection

Previously used in Drosophila, not tested in Sf9 cells.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo. Wright et al (Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):55-60. Epub 2008 Dec 30. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31900" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with