Gene/insert name: Pygopus
Species:
D. melanogaster (fly)
Entrez Gene:
pygo
(Dmel_CG11518, BEST:LD21971, CG11518, Dm Pygo, Dmel\CG11518, Pygo, anon-WO02077023.1, dPygo, gam, pNB5.2, pyg, pypo)
Fusion protein or tag: V5
Terminal: C terminal on backbone
Fusion protein or tag: 6xHIS
Terminal: C terminal on backbone
Vector backbone:
pMT-V5/HISSearch Vector Database )
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression
Backbone size w/o insert (bp):
3538
Promoter: P-MT (Metallothionein)
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
Metallothionein
List of Sequencing Primers
3' sequencing primer:
EBV reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
See comments
Sequence:
View sequences (1)
Principal Investigator:
Robert Tjian
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
31900" in your Materials and Methods section.
Blasticidin OR Hygromycin selection
Previously used in Drosophila, not tested in Sf9 cells.