pMT-Pygopus
(Plasmid #31900)

• Depositing Lab: Robert Tjian
• Publication: Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):55-60. Epub 2008 Dec 30.

Backbone

• Vector backbone: pMT-V5/HIS
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3538
• Vector type: Insect Expression
• Selectable markers: See comments

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pygopus
• Species: D. melanogaster (fly)
• Entrez Gene: pygo (a.k.a. Dmel_CG11518, BEST:LD21971, CG11518, Dm Pygo, Dmel\CG11518, Pygo, anon-WO02077023.1, dPygo, gam, pNB5.2, pyg, pypo)
• Promoter: P-MT (Metallothionein)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: Metallothionein
• 3′ sequencing primer: EBV reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Blasticidin OR Hygromycin selection

Previously used in Drosophila, not tested in Sf9 cells.