Addgene - pYES2.1-ScDcr1 Plasmid Data

Plasmid 32036: pYES2.1-ScDcr1

Gene/insert name:
Drc1
Species:
S. castellii
Vector backbone:
pYES2.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5800
Promoter:
GAL1
Cloning site 5':
unknown
Site destroyed during cloning:
Unknown
Cloning site 3':
unknown
Site destroyed during cloning:
Unknown
5' sequencing primer:
Gal1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
URA3
Sequence: View sequences (1)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The inside-out mechanism of dicers from budding yeasts. Weinberg et al (Cell. 2011 Jul 22;146(2):262-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32036" in your Materials and Methods section.