Addgene - MCV-HF Plasmid Data

Plasmid 32057: MCV-HF

Gene/insert name:
MCV-HF genome
Insert size:
5387
Species:
Merkel cell polyomavirus
GenBank ID:
JF813003
Vector backbone:
pJ241
(Search Vector Database)
Backbone manufacturer:
DNA2.0
Backbone size w/o insert (bp):
2755
Promoter:
none from vector. Insert contains native promoter from viral genome encompassed in the viral non-coding control region.
Cloning site 5':
BsrFI
Site destroyed during cloning:
No
Cloning site 3':
BsrFI
Site destroyed during cloning:
No
5' sequencing primer:
NA List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Patrick Moore
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cellular and viral factors regulating merkel cell polyomavirus replication. Feng et al (PLoS One. 2011;6(7):e22468. Epub 2011 Jul 22. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32057" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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