MCV-HF
(Plasmid #32057)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    MCV-HF genome
  • Species
    Merkel cell polyomavirus
  • Insert Size (bp)
    5387
  • GenBank ID
    JF813003
  • Promoter none from vector. Insert contains native promoter from viral genome encompassed in the viral non-coding control region.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrFI (not destroyed)
  • 3′ cloning site BsrFI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCV-HF was a gift from Patrick Moore (Addgene plasmid # 32057)
  • For your References section:

    Cellular and viral factors regulating merkel cell polyomavirus replication. Feng H, Kwun HJ, Liu X, Gjoerup O, Stolz DB, Chang Y, Moore PS. PLoS One. 2011;6(7):e22468. Epub 2011 Jul 22. 10.1371/journal.pone.0022468 PubMed 21799863