Addgene - pwB2b Plasmid Data

Plasmid 32094: pwB2b

Gene/Insert 1
Gene/insert name:
BKV VP1
Alt name:
BK polyomavirus
Alt name:
BKPyV
Insert size:
1088
Species:
virus
Mutation:
codon modified
Gene/Insert 2
Gene/insert name:
BKV VP2
Insert size:
1055
Species:
virus
Mutation:
codon modified
Vector backbone:
pGwf
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5338
Cloning information for Gene/Insert 1
Cloning method:
Gateway Cloning
5' sequencing primer:
attR1 List of Sequencing Primers
3' sequencing primer:
attR2
Bacterial resistance(s)
Bleocin (Zeocin)
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Christopher Buck
Terms and Licenses:
MTA

Comments:

Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Glycosaminoglycans and sialylated glycans sequentially facilitate merkel cell polyomavirus infectious entry. Schowalter et al (PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32094" in your Materials and Methods section.

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