|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32101||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 6700
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneMt1 (a.k.a. MT-I, Mt-1)
- Promoter P-lac
/ Fusion Protein
- MBP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XmnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer MBP_F (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe metallothionein (MT) gene was amplified from pET-3d-MT (a gift from the Winge laboratory, University of Utah), which contains the MT gene within the pET-3d vector (Novagen, Madison, WI).
Terms and Licenses
The metallothionein (MT) gene was amplified with the forward primer (5'-CTCGGGATCGAGGGAAGGATTTCAAGA TATACCATGGACCCC-3'), which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (5'-GACT CTAGAGGATCCTAGGCACAGCACGTG-3' ), which contains the MT gene stop codon and a subsequent BamHI site. Both the PCR product and pMAL-c2x vector were digested with XmnI and BamHI and were later ligated together to generate the final plasmid. In addition to this plasmid Addgene offers versions with 2 copies (32102), 3 copies (32115) and 4 copies (32100) of MT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMAL-c2x-MT1 was a gift from David DeRosier & Chris Mercogliano & Cristina Risco Ortiz (Addgene plasmid # 32101 ; http://n2t.net/addgene:32101 ; RRID:Addgene_32101)
For your References section:Concatenated metallothionein as a clonable gold label for electron microscopy. Mercogliano CP, DeRosier DJ. J Struct Biol. 2007 Oct;160(1):70-82. Epub 2007 Jul 10. 10.1016/j.jsb.2007.06.010 PubMed 17692533