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413-Met25 Kar2 TorsinA K108A GFP
(Plasmid #32125)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    TorsinA
  • Species
    H. sapiens (human)
  • Mutation
    K108A
  • Entrez Gene
    TOR1A (a.k.a. DQ2, DYT1)
  • Tags / Fusion Proteins
    • Kar2 (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13-rev
  • 3′ sequencing primer M13-F20
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    413-Met25 Kar2 TorsinA K108A GFP was a gift from Susan Lindquist (Addgene plasmid # 32125)
  • For your References section:

    TorsinA and the TorsinA-Interacting Protein Printor Have No Impact on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast. Valastyan JS, Lindquist S. PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26. 10.1371/journal.pone.0022744 PubMed 21818381