Addgene - 413-Met25 Kar2 TorsinADE K108A GFP Plasmid Data

Plasmid 32126: 413-Met25 Kar2 TorsinADE K108A GFP

Gene/insert name:
TorsinA
Species:
H. sapiens (human)
Fusion protein or tag:
Kar2
Terminal:
N terminal on insert
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
deletion of E at 302 or 303, K108A
Vector backbone:
413-Met25
(Search Vector Database)
Vector type:
Yeast Expression
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
m13-r List of Sequencing Primers
3' sequencing primer:
m13-f
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
HIS3
Sequence: View sequences (3)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TorsinA and the TorsinA-Interacting Protein Printor Have No Impact on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast. Valastyan et al (PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32126" in your Materials and Methods section.