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LHP306 - Ub - no Lys
(Plasmid #32150)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32150 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTER62
  • Backbone manufacturer
    Mike Ellison - University of Alberta
  • Modifications to backbone
    The c-myc epitope tag was removed
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthetic yeast Ubiquitin
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R
  • Entrez Gene
    UBA1 (a.k.a. YKL210W)
  • Entrez Gene
    UBA2 (a.k.a. YDR390C, UAL1)
  • Promoter CUP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CUP1 forward
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ub-no Lys; CUP1-Ub K6R, K11R, K27R, K29R, K33A, K48R, K63R (all Lys Changed to Arg); BamHI-BglII UBI fragment from YEp96 (D. Ecker, U PENN) was cloned into pTER62 (Mike Ellison, University of Alberta) cut with BamHI and BglII.

2 µm S. cerevisiae origin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP306 - Ub - no Lys was a gift from Linda Hicke (Addgene plasmid # 32150 ; http://n2t.net/addgene:32150 ; RRID:Addgene_32150)
  • For your References section:

    A function for monoubiquitination in the internalization of a G protein-coupled receptor. Terrell J, Shih S, Dunn R, Hicke L. Mol Cell. 1998 Jan;1(2):193-202. 10.1016/S1097-2765(00)80020-9 PubMed 9659916