LHP306 - Ub - no Lys
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32150||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMike Ellison - University of Alberta
Modifications to backboneThe c-myc epitope tag was removed
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CUP1 forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Ub-no Lys; CUP1-Ub K6R, K11R, K27R, K29R, K33A, K48R, K63R (all Lys Changed to Arg); BamHI-BglII UBI fragment from YEp96 (D. Ecker, U PENN) was cloned into pTER62 (Mike Ellison, University of Alberta) cut with BamHI and BglII.
2 µm S. cerevisiae origin
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LHP306 - Ub - no Lys was a gift from Linda Hicke (Addgene plasmid # 32150 ; http://n2t.net/addgene:32150 ; RRID:Addgene_32150)
For your References section:A function for monoubiquitination in the internalization of a G protein-coupled receptor. Terrell J, Shih S, Dunn R, Hicke L. Mol Cell. 1998 Jan;1(2):193-202. 10.1016/S1097-2765(00)80020-9 PubMed 9659916