|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32172||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
MutationStop codon introduced at aa 378
Entrez GeneSTE2 (a.k.a. YFL026W)
- Promoter STE2
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer M13_puc_Fwd
- 3′ sequencing primer M13_puc_Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
STE2 ORF with a stop codon introduced at aa378. The 1.6kB STE2 378Stop -encoding HindIII fragment was subcloned from LHP147 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI(upstream)/PstI fragment and ligating in the 2KB EcoRI/PstI fragment from pJR3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LHP428-YCplac33-STE2-378Stop was a gift from Linda Hicke (Addgene plasmid # 32172 ; http://n2t.net/addgene:32172 ; RRID:Addgene_32172)