Addgene - LHP1480 - TOPO-TAP-tag Plasmid Data

Plasmid 32229: LHP1480 - TOPO-TAP-tag

Gene/insert name:
TAP tag
Vector backbone:
TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Cloning
Promoter:
P-lac
Cloning method:
TOPO Cloning
5' sequencing primer:
M13 reverse List of Sequencing Primers
3' sequencing primer:
M13 forward
Bacterial resistance(s)
Ampicillin and Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Linda Hicke
Terms and Licenses:
MTA

Comments:

The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32229" in your Materials and Methods section.