LHP1480 - TOPO-TAP-tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32229||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
Gene/Insert nameTAP tag
- Promoter P-lac
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LHP1480 - TOPO-TAP-tag was a gift from Linda Hicke (Addgene plasmid # 32229)