Plasmid 32254: TAL010
  • TAL010

  • S (Type I N-terminal - T/NG)

  • α / SNG / T

  • 123

  • pUC57-ΔBsaI
    (Search Vector Database)

  • 2708

  • Silent valine mutation (C->G) at position 1794 to destroy alternate BsaI restriction site in pUC57

  • BbsI

  • Yes

  • BamHI

  • No

  • OK163 List of Sequencing Primers

  • Ampicillin

  • XL1 Blue

  • 37

  • High Copy

  • View sequences (2)
  • Keith Joung


Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32254" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with